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CA2 / Anti-Beta carbonic anhydrase 2, chloroplastic antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY1518S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

Beta carbonic anhydrase 2 is involved in reversible hydration of carbon dioxide, and ensures the supply of bicarbonate for pep carboxylase. In Arabidopsis three CA proteins are present, termed CA1 (AT3G01500), CA2 and CA3 (AT1G23730).

  Synonyms:

CA2, BETA CA2, BETA CARBONIC ANHYDRASE 2, CA18, CARBONIC ANHYDRASE 18, CARBONIC ANHYDRASE 2

  Immunogen:

KLH-conjugated synthetic peptide of CA2 derived from Arabidopsis thaliana AT5G14740.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at arashare01@qq.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:5000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

37 / 28 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica napusBrassica rapa
For more species homologues information, please contact tech support at arashare01@qq.com.

 

Application Example

5 μl total chloroplast protein from Arabidopsis thaliana leaf.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:5000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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