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CAT3 / Anti-Catalase-3 antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY1535S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

Catalase is one of the most important antioxidant enzymes that catalyze the decomposition of H2O2. CAT3 has been found to interact with several proteins, such as nucleoside diphosphate kinase, NDK1 (AT4G09320), class 3 sucrose-nonfermenting 1-related kinase, SOS2 (AT5G35410), and LESION SIMULATING DISEASE1, LSD1 (At4g20380).

  Synonyms:

CAT3, ATCAT3, CATALASE 3, SEN2, SENESCENCE 2

  Immunogen:

KLH-conjugated synthetic peptide of CAT3 derived from Arabidopsis thaliana AT1G20620.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at arashare01@qq.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

57 / 55 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Brassica napus.
For more species homologues information, please contact tech support at arashare01@qq.com.

 

Application Example

Lane 1: 6 μg stromal protein from Arabidopsis thaliana leaf. 
Lane 2: 12 μg stromal protein from Arabidopsis thaliana leaf. 
Electrophoresis: 12% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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