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UCP1 / Anti-Mitochondrial uncoupling protein 1, C-terminal antibody
价格:¥2540.00 ¥3180.00
积分:150 分
货号:PHY1574A
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

In plants, members of the UCP family have been associated with several physiological roles, including thermogenesis regulation, abiotic stress responses and climacteric increases in respiration. And some researches show that AtUCP1 responses to abiotic stresses.

  Synonyms:

UCP1, ARABIDOPSIS THALIANA PLANT UNCOUPLING MITOCHONDRIAL PROTEIN 1, ARABIDOPSIS THALIANA UNCOUPLING PROTEIN 1, ATPUMP1, ATUCP1, PLANT UNCOUPLING MITOCHONDRIAL PROTEIN 1, PUMP1, UCP, UNCOUPLING PROTEIN 1

  Immunogen:

KLH-conjugated synthetic peptide derived from C-terminal of UCP1 protein in Arabidopsis thaliana AT3G54110.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150µl of 0.01 M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

33 / 27 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

For more species homologues information, please contact tech support at arashare01@qq.com.

 

Application Example

Lane 1 : 10 μg mitochondria protein from Arabidopsis thaliana leaf.
Electrophoresis:15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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