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RPT5A/B / Anti-26S proteasome regulatory subunit 6A homolog A/B antibody
价格:¥2540.00 ¥3180.00
积分:150 分
货号:PHY1747A
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

RPT5a (Regulatory Particle 5a), one of the six AAA-ATPases of the proteasome regulatory particle. It’s essential for gametophyte development. In Arabidopsis, the RPT5 subunit is encoded by two highly homologous genes, RPT5a and RPT5b. RPT5a and RPT5b show accession-dependent functional redundancy.

  Synonyms:

RPT5A/B, ATS6A.2, REGULATORY PARTICLE TRIPLE-A ATPASE 5A, RPT5A, 26S PROTEASOME AAA-ATPASE SUBUNIT RPT5B, RPT5B

  Immunogen:

KLH-conjugated synthetic peptide of RPT5A/B derived from Arabidopsis thaliana AT3G05530, AT1G09100.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150µl of 0.01 M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

47 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

For more species homologues information, please contact tech support at arashare01@qq.com.

 

Application Example

Lane 1: 30 μg total protein from Arabidopsis thaliana leaf. 
Lane 2: 60 μg total protein from Arabidopsis thaliana leaf. 
Lane 3: 15 μg soluble protein from Arabidopsis thaliana leaf. 
Lane 4: 30 μg soluble protein from Arabidopsis thaliana leaf. 
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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