Product Information
Description: | Rabbit polyclonal antibody |
Background: | Arabidopsis thaliana, like human, has two tightly regulated N-myristoyltransferase (NMT) genes, NMT1 (AT5G57020) and NMT2 (AT2G44170). The AtNMT1 expression profile indicated ubiquity in roots, stem, leaves, flowers, and siliques (approximately 1.7 kb transcript and approximately 50 kDa immunoreactive polypeptide) but a greater level in the younger tissue, which are developmentally very active. |
Synonyms: | NMT1, ARABIDOPSIS THALIANA MYRISTOYL-COA:PROTEIN N-MYRISTOYLTRANSFERASE, ATNMT1, MYRISTOYL-COA:PROTEIN N-MYRISTOYLTRANSFERASE |
Immunogen: | KLH-conjugated synthetic peptide of NMT1 derived from Arabidopsis thaliana AT5G57020. |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Serum |
Reconstitution: | Reconstitution with 150µl of sterile water. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Recommended Dilution: | Western Blot (1:1000-1:2000) |
Expected/apparent MW: | 50 kDa |
Confirmed Reactivity: | Arabidopsis thaliana |
Predicted Reactivity: | Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Populus trichocarpa, Brassica napus, Glycine max, Medicago truncatula, and 80-99% homologues with the sequence in Vitis vinifera, Spinacia oleracea, Solanum lycopersicum, Oryza sativa Japonica Group, Sorghum bicolor, Zea mays. |
Application Example
10 μg mitochondria protein from Arabidopsis thaliana leaf. |