Product Information
Description: | Rabbit polyclonal antibody |
Background: | SPO11 is a key protein for promoting meiotic recombination, by generating chromatin locus- and timing-specific DNA double-strand breaks (DSBs). AtSPO11-1 is one of the three Arabidopsis homologues of the archaeal DNA topoisomerase VIA subunit (topo VIA). |
Synonyms: | SPO11-1, ATSPO11-1, SPORULATION 11-1 |
Immunogen: | KLH-conjugated synthetic peptide of SPO11-1 derived from Arabidopsis thaliana AT3G13170. |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Serum |
Reconstitution: | Reconstitution with 150 µl of sterile water. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Recommended Dilution: | Western Blot (1:1000-1:2000) |
Expected/apparent MW: | 42 / 40 kDa |
Confirmed Reactivity: | Arabidopsis thaliana |
Predicted Reactivity: | Among species analyzed, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Brassica rapa, Brassica napus, Gossypium raimondii, Solanum lycopersicum, Spinacia oleracea, Solanum tuberosum, Nicotiana tabacum. |
Application Example
4.5 μg nuclear protein from Arabidopsis thaliana. |