Product Information
Description: | Rabbit polyclonal antibody |
Background: | CBP20 is a nuclear cap-binding protein that forms a heterodimeric complex with ABH1 (AT2G13540) and is likely to participate in RNA metabolism. It recognizes and binds capped RNAs (m7GpppG-capped RNA) but requires ABH1/CBP80 to stabilize the movement of its N-terminal loop and lock the CBC into a high affinity cap-binding state with the cap structure. |
Synonyms: | CBP20, ATCBP20, CAP-BINDING PROTEIN 20 |
Immunogen: | KLH-conjugated synthetic peptide derived from N-terminal section of CBP20 protein in Arabidopsis thaliana AT5G44200. |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Serum |
Reconstitution: | Reconstitution with 150µl of sterile water. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Recommended Dilution: | Western Blot (1:1000-1:2000) |
Expected/apparent MW: | 30 kDa |
Confirmed Reactivity: | Arabidopsis thaliana |
Predicted Reactivity: | Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica napus, and 80-99% homologues with the sequence in Medicago truncatula, Glycine max, Populus trichocarpa, Solanum tuberosum, Solanum lycopersicum, Nicotiana tabacum, Spinacia oleracea, Zea mays, Vitis vinifera, Hordeum vulgare, Sorghum bicolor, Oryza sativa Japonica Group. |
Application Example
Lane 1: 10 μg cytosolic protein from Arabidopsis thaliana leaf. |