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CBP20 / Anti-Nuclear cap-binding protein subunit 2 antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY2015S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

CBP20 is a nuclear cap-binding protein that forms a heterodimeric complex with ABH1 (AT2G13540) and is likely to participate in RNA metabolism. It recognizes and binds capped RNAs (m7GpppG-capped RNA) but requires ABH1/CBP80 to stabilize the movement of its N-terminal loop and lock the CBC into a high affinity cap-binding state with the cap structure.

  Synonyms:

CBP20, ATCBP20, CAP-BINDING PROTEIN 20

  Immunogen:

KLH-conjugated synthetic peptide derived from N-terminal section of CBP20 protein in Arabidopsis thaliana AT5G44200.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at arashare01@qq.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

30 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica napus, and 80-99% homologues with the sequence in Medicago truncatula, Glycine max, Populus trichocarpa, Solanum tuberosum, Solanum lycopersicum, Nicotiana tabacum, Spinacia oleracea, Zea mays, Vitis vinifera, Hordeum vulgare, Sorghum bicolor, Oryza sativa Japonica Group.
For more species homologues information, please contact tech support at arashare01@qq.com.

 

Application Example

Lane 1: 10 μg cytosolic protein from Arabidopsis thaliana leaf. 
Lane 2: 20 μg cytosolic protein from Arabidopsis thaliana leaf. 
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.