返回首页 登录 注册 购物车
关于我们 通知公告 服务指南 联系我们
试剂耗材频道
搜产品 敬请关注公众号
总站导航 AraShare PlantShare ExpShare 植科头条 学术讲堂 求职招聘 仪器设备 试剂耗材 技术服务 试剂耗材 植物抗体 拟南芥抗体
RPS6 / Anti-40S ribosomal protein S6-1 antibody
价格:¥2860.00 ¥3180.00
积分:150 分
货号:PHY2025A
数量:999
- +
加入购物车 立即购买 收藏此商品

Product Information

  Description:

Rabbit polyclonal antibody

  Background:

RPS6A and RPS6B (AT5G10360) are fully redundant and essential during gametogenesis. It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.

  Synonyms:

RPS6, ATRPS6, RIBOSOMAL PROTEIN S6, RPS6A

  Immunogen:

KLH-conjugated synthetic peptide derived from N-terminal of RPS6 protein in Arabidopsis thaliana AT4G31700.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150µl of 0.01 M sterile PBS.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

28 / 30 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Glycine max, and 80-99% homologues with the sequence in Spinacia oleracea, Zea mays.
The sequence of the synthetic peptide used for immunization is 94% homologues with the sequence in RPS6B (AT5G10360).
For more species homologues information, please contact tech support at arashare01@qq.com.

 

Application Example

Lane 1: 12.5 μg cytosolic protein from Arabidopsis thaliana leaf.
Lane 2: 5 μg nuclear protein from Arabidopsis thaliana.
Lane 3: 20 μg total protein from Arabidopsis thaliana leaf.
Lane 4: 12 μg stromal protein from Arabidopsis thaliana leaf.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


猜你喜欢 CSD2 / Anti-Ch...
¥ 2540.00 ¥ 3180.00
AtpB / Anti-Be...
¥ 3100.00 ¥ 3880.00
Arf1 /Anti-ADP...
¥ 2310.00 ¥ 2890.00
Actin / Anti-A...
¥ 3100.00 ¥ 3880.00
CSD2 / Anti-Ch...
¥ 2540.00 ¥ 3180.00
AtpB / Anti-Be...
¥ 3100.00 ¥ 3880.00
Arf1 /Anti-ADP...
¥ 2310.00 ¥ 2890.00
Actin / Anti-A...
¥ 3100.00 ¥ 3880.00