PEPC1 is one of four Arabidopsis phosphoenolpyruvate carboxylase proteins. It plays an important role in carbon and nitrogen metabolism.
PEPC1, PEPCase, PEP carboxylase, ATPPC1
KLH-conjugated synthetic peptide derived from C-terminal of PEPC1 in Arabidopsis thalianaAT1G53310.
Serum Peptide affinity form antibody available upon request at email@example.com.
Reconstitution with 150µl of sterile water. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
For more species homologues information, please contact tech support at firstname.lastname@example.org.
Lane 1: 10 μg cytosolic protein from Arabidopsis thaliana leaf. Lane 2: 20 μg cytosolic protein from Arabidopsis thaliana leaf. Electrophoresis: 10% SDS-PAGE Transfer: blotting to NC (nitrocellulose) membrane for 1h. Blocking: 5% skim milk at RT or 4℃ for 1h. Primary antibody: 1:1000 dilution overnight at 4℃. Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000) Detection:using chemiluminescence substrate and image were captured with CCD camera.