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alpha-TIP / Anti-Aquaporin TIP3-1 antibody
价格:¥2540.00 ¥3180.00
积分:150 分
货号:PHY2096A
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

Tonoplast intrinsic proteins (TIPs), which belong to the major intrinsic protein family, are members of plant aquaporins (AQPs) and are localized in the membrane of the vacuole. The TIP family consists of five subgroups, namely TIP1 (γ-TIP), TIP2 (δ-TIP), TIP3 (α-TIP and β-TIP), TIP4 (ε-TIP), and TIP5 (ξ-TIP). TIP3;1 and TIP3;2 are specifically expressed during seed maturation and localized to the seed protein storage vacuole membrane.

  Synonyms:

alpha-TIP, ALPHA-TIP, ALPHA-TONOPLAST INTRINSIC PROTEIN, TIP3;1

  Immunogen:

KLH-conjugated synthetic peptide of alpha-TIP derived from Arabidopsis thaliana AT1G73190.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150µl of 0.01 M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

28 / 24 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

For more species homologues information, please contact tech support at arashare01@qq.com.

 

Application Example

20 μg total protein from Arabidopsis thaliana leaf.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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