Product Information
Description: | Rabbit polyclonal antibody |
Background: | Cytochrome c oxidase is the last enzyme in the respiratory electron transport chain of mitochondria and it is also called Complex IV. Cytochrome c oxidase receives an electron from each of four cytochrome c molecules, and transfers them to one oxygen molecule, converting molecular oxygen to two molecules of water. In higher plants mitochondria, Complex IV processes 14 subunits. COX Vb (AT3G15640/AT1G80230) is one subunit of the Complex IV. |
Synonyms: | COX Vb, COX5B-1, COX5B |
Immunogen: | KLH-conjugated synthetic peptide of COX Vb derived from Arabidopsis thaliana AT3G15640, AT1G80230. |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Serum |
Reconstitution: | Reconstitution with 150 µl of sterile water. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Recommended Dilution: | Western Blot (1:1000-1:2000) |
Expected/apparent MW: | 19 kDa |
Confirmed Reactivity: | Arabidopsis thaliana |
Predicted Reactivity: | Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Vitis vinifera, Spinacia oleracea, Brassica napus, Glycine max, Populus trichocarpa, Medicago truncatula, Zea mays, and 80-99% homologues with the sequence in Cucumis sativus. |
Application Example
Lane 1: 15 μg mitochondria protein from Arabidopsis thaliana leaf. |