Product Information
Description: | Rabbit polyclonal antibody |
Background: | Alternative oxidases (AOX) are quinol oxidases located in the inner mitochondrial membrane of plants. They function as terminal oxidases in the alternate electron transport pathway, oxidizing ubiquinone to reduce oxygen to water. AOX isoenzymes are including AOX1A(AT3G22370), AOX1B(AT3G22360), AOX1C(AT3G27620) and AOX1D(AT1G32350) from Arabidopsis. |
Synonyms: | AOX1 |
Immunogen: | KLH-conjugated synthetic peptide derived from C-terminal of AOX1 protein in Arabidopsis thaliana AT3G22370, AT3G22360. |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Immunogen affinity purified |
Reconstitution: | Reconstitution with 150µl of 0.01 M sterile PBS. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Recommended Dilution: | Western Blot (1:1000-1:2000) |
Expected/apparent MW: | 40kDa(AT3G22370), 37 / 33 kDa(AT3G22360) |
Confirmed Reactivity: | Arabidopsis thaliana |
Predicted Reactivity: | Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica napus, and 80-99% homologues with the sequence in Oryza sativa Japonica Group. |
Application Example
15 μg mitochondria protein from Arabidopsis thaliana leaf. |