Product Information
Description: | Rabbit polyclonal antibody |
Background: | Arabidopsis V-ATPase has three B subunits (AtVAB1, AtVAB2, and AtVAB3), which share 97.27% sequence identity and have two potential actin-binding sites, indicating that these AtVABs may have crucial functions in actin cytoskeleton remodeling and plant cell development. |
Synonyms: | VAB1/2/3, ATVAB1/2/3, V-ATPASE B SUBUNIT 1/2/3 |
Immunogen: | KLH-conjugated synthetic peptide of VAB1/2/3 derived from Arabidopsis thaliana AT1G76030, AT4G38510, AT1G20260. |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Serum |
Reconstitution: | Reconstitution with 150 µl of sterile water. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Recommended Dilution: | Western Blot (1:1000-1:2000) |
Expected/apparent MW: | 54 kDa |
Confirmed Reactivity: | Arabidopsis thaliana |
Predicted Reactivity: | Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Hordeum vulgare, Gossypium raimondii, Oryza sativa Japonica Group, Zea mays, Vitis vinifera, Sorghum bicolor, Populus trichocarpa, Medicago truncatula, Glycine max, Brassica rapa, Setaria viridis, Cucumis sativus, Brassica napus, Triticum aestivum, Spinacia oleracea, and 80-99% homologues with the sequence in Nicotiana tabacum, Solanum lycopersicum, Solanum tuberosum, Physcomitrella patens. |
Application Example
20 μg total protein from Arabidopsis thaliana leaf. |