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PsbO / Anti-PsbO subunit of oxygen evolving complex of Photosystem II, C-terminal antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY2255S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

PsbO is an extrinsic subunit of photosystem II, which has been proposed to play a central role in stabilization of the catalytic manganese cluster. In Arabidopsis thaliana, the PsbO proteins are encoded by two genes: psbO1 (AT5G66570) and psbO2 (AT3G50820). PsbO1 is the major isoform in Arabidopsis.

  Synonyms:

PsbO, OEE1, 33 kDa subunit of oxygen evolving system of photosystem II, 33 kDa thylakoid membrane protein, Manganese-stabilizing protein 1, MSP-1, OEC 33 kDa subunit, 33 kDa of the oxygen evolving complex (OEC) of PSII, Oxygen-evolving enhancer protein 1, chloroplastic

  Immunogen:

KLH-conjugated synthetic peptide derived from C-terminal of PsbO protein in Arabidopsis thalianaAT5G66570, AT3G50820.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at arashare01@qq.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

35 / 27 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica napusBrassica rapa, and 80-99% homologues with the sequence in Spinacia oleracea, Nicotiana tabacumVitis viniferaSolanum lycopersicumSolanum tuberosumOryza sativa Japonica GroupGossypium raimondiiPopulus trichocarpa.
For more species homologues information, please contact tech support at arashare01@qq.com.

 

 Application Example

Lane 1: 12 μg stromal protein from Arabidopsis thaliana leaf.
Lane 2: Thylakoid membrane protein from Arabidopsis thaliana leaf containing 5 μg of chlorophyll.
Lane 3: 10 μl total chloroplast protein from Arabidopsis thaliana leaf.
Lane 4: 18 μg total protein from Arabidopsis thaliana leaf.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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