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PsbO / Anti-PsbO subunit of oxygen evolving complex of Photosystem II, C-terminal antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY2255S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

PsbO is an extrinsic subunit of photosystem II, which has been proposed to play a central role in stabilization of the catalytic manganese cluster. In Arabidopsis thaliana, the PsbO proteins are encoded by two genes: psbO1 (AT5G66570) and psbO2 (AT3G50820). PsbO1 is the major isoform in Arabidopsis.

  Synonyms:

PsbO, OEE1, 33 kDa subunit of oxygen evolving system of photosystem II, 33 kDa thylakoid membrane protein, Manganese-stabilizing protein 1, MSP-1, OEC 33 kDa subunit, 33 kDa of the oxygen evolving complex (OEC) of PSII, Oxygen-evolving enhancer protein 1, chloroplastic

  Immunogen:

KLH-conjugated synthetic peptide derived from C-terminal of PsbO protein in Arabidopsis thalianaAT5G66570, AT3G50820.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at arashare01@qq.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

35 / 27 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica napus, Brassica rapa, and 80-99% homologues with the sequence in Spinacia oleracea, Nicotiana tabacum, Vitis vinifera, Solanum lycopersicum, Solanum tuberosum, Oryza sativa Japonica Group, Gossypium raimondii, Populus trichocarpa.
For more species homologues information, please contact tech support at arashare01@qq.com.

 

 Application Example

Lane 1: 12 μg stromal protein from Arabidopsis thaliana leaf.
Lane 2: Thylakoid membrane protein from Arabidopsis thaliana leaf containing 5 μg of chlorophyll.
Lane 3: 10 μl total chloroplast protein from Arabidopsis thaliana leaf.
Lane 4: 18 μg total protein from Arabidopsis thaliana leaf.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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