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PAM68 / Anti-Photosynthesis Affected Mutant 68 antibody
价格:¥2540.00 ¥3180.00
积分:150 分
货号:PHY2289A
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

PAM68 is a conserved integral membrane protein found in cyanobacterial and eukaryotic thylakoids and interacts with several PSII core proteins and known PSII assembly factors, such as D1, D2, and so on. PAM68 proteins promote early steps in PSII biogenesis by associating with an early intermediate complex that might contain D1 and the assembly factor LPA1.

  Synonyms:

PAM68, PHOTOSYNTHESIS AFFECTED MUTANT 68

  Immunogen:

KLH-conjugated synthetic peptide of PAM68 derived from Arabidopsis thaliana AT4G19100.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150 µl of 0.01 M sterile PBS.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Applications: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected Results: 

24 / 19 kDa

 Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

For more species homologues information, please contact tech support at arashare01@qq.com.

 

Application Example

80 μg total chloroplast protein from Arabidopsis thaliana leaf.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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