Product Information
Description: | Rabbit polyclonal antibody |
Background: | NADP-dependent malic enzyme (NADP-ME) catalyzes the oxidative decarboxylation of malate to generate pyruvate, CO2 and NADPH. Arabidopsis thaliana possesses three cytosolic (NADP-ME1-3) and one plastidic NADP-ME isoforms (NADP-ME4, AT1G79750). NADP-ME2 (AT5G11670) is responsible for most of the NADP-ME activity measured in mature organs and has been involved in sugar metabolism in veins and in the oxidative burst triggered by hemibiotrophic fungal pathogen infection. |
Synonyms: | NADP-ME2, ARABIDOPSIS THALIANA NADP-MALIC ENZYME 2, ATNADP-ME2, NADP-MALIC ENZYME 2 |
Immunogen: | KLH-conjugated synthetic peptide derived from C-terminal section of NADP-ME2 protein in Arabidopsis thaliana AT5G11670. |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Serum |
Reconstitution: | Reconstitution with 150µl of sterile water. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Recommended Dilution: | Western Blot (1:1000-1:2000) |
Expected/apparent MW: | 64 kDa |
Confirmed Reactivity: | Arabidopsis thaliana |
Predicted Reactivity: | Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Brassica napus. |
Application Example
lane 1: 12 μg stromal protein from Arabidopsis thaliana leaf. |