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NADP-ME2 / Anti-NADP-dependent malic enzyme 2 antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY2303S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

NADP-dependent malic enzyme (NADP-ME) catalyzes the oxidative decarboxylation of malate to generate pyruvate, CO2 and NADPH. Arabidopsis thaliana possesses three cytosolic (NADP-ME1-3) and one plastidic NADP-ME isoforms (NADP-ME4, AT1G79750). NADP-ME2 (AT5G11670) is responsible for most of the NADP-ME activity measured in mature organs and has been involved in sugar metabolism in veins and in the oxidative burst triggered by hemibiotrophic fungal pathogen infection.

  Synonyms:

NADP-ME2, ARABIDOPSIS THALIANA NADP-MALIC ENZYME 2, ATNADP-ME2, NADP-MALIC ENZYME 2

  Immunogen:

KLH-conjugated synthetic peptide derived from C-terminal section of NADP-ME2 protein in Arabidopsis thaliana AT5G11670.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at arashare01@qq.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

64 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Brassica napus.
For more species homologues information, please contact tech support at arashare01@qq.com.

 

 Application Example

lane 1: 12 μg stromal protein from Arabidopsis thaliana leaf.
lane 2: 30 μg cytosolic protein from Arabidopsis thaliana leaf.
lane 3: 10 μg cytosolic protein from Arabidopsis thaliana leaf.
lane 4: 18 μg total protein from Arabidopsis thaliana leaf.
Electrophoresis: 12% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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