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GPX1/7 / Anti-GLUTATHIONE PEROXIDASE 1/7 antibody
价格:¥2540.00 ¥3180.00
积分:150 分
货号:PHY2318A
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

The plant glutathione peroxidase (GPX) family consists of multiple isoenzymes with distinct subcellular locations which exhibit different tissue-specific expression patterns and environmental stress responses. Glutathione peroxidase-like enzymes (GPXLs) constitute a family of eight peroxidases in Arabidopsis thaliana.

  Synonyms:

GPX1/7, ATGPX1/7, GLUTATHIONE PEROXIDASE 1/7, GPXL1/7

  Immunogen:

KLH-conjugated synthetic peptide of GPX1/7 derived from Arabidopsis thaliana AT2G25080, AT4G31870.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150µl of 0.01 M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

26 / 18 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Oryza sativa Japonica GroupGossypium raimondiiCucumis sativusBrassica rapaBrassica napusMedicago truncatulaSetaria viridisHordeum vulgare subsp. vulgare, and 80-99% homologues with the sequence in Zea maysGlycine maxSorghum bicolorVitis viniferaNicotiana tabacumPopulus trichocarpaSpinacia oleraceaSolanum lycopersicumSolanum tuberosum
For more species homologues information, please contact tech support at arashare01@qq.com

 

Application Example

40 μg total protein from Arabidopsis thaliana leaf.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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