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PsbR / Anti-PsbR subunit of Photosystem II antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY2395S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

PsbR is the subunit of photosystem II (PSII) with a molecular mass of 10 kDa. This subunit appears to be involved in the stable assembly of PSII, particularly that of the oxygen-evolving complex subunit PsbP. Mutants defective in accumulation of PsbR have reduced amounts of subunits PsbP and PsbQ in PSII.

  Synonyms:

PsbR, Photosystem II 10 kDa polypeptide, chloroplastic, photosystem II subunit R

  Immunogen:

KLH-conjugated synthetic peptide derived from central section of PsbR protein in Arabidopsis thaliana AT1G79040.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at arashare01@qq.com

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Applications: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected Results: 

15 / 11 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Populus trichocarpa, Brassica napus, Brassica rapa, Spinacia oleracea, Gossypium raimondii, Nicotiana tabacum, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Zea mays, and 80-99% homologues with the sequence in Glycine max, Cucumis sativus, Oryza sativa Japonica Group, Vitis vinifera, Medicago truncatula, Setaria viridis, Hordeum vulgare, Triticum aestivum, Solanum tuberosum, Chlamydomonas reinhardtii.
For more species homologues information, please contact tech support at arashare01@qq.com

 

Application Example

1-3 is thylakoid membrane protein from WT of Arabidopsis thaliana leaf containing 1.25 μg, 2.5 μg, and 5 μg of chlorophyll, respectively.
Electrophoresis: Tricine-SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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