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PsbP / Anti-PsbP subunit of oxygen evolving complex of Photosystem II antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY2363S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

PsbP is a 23 kD extrinsic subunit of the oxygen evolving complex of Photosystem II. PsbP participates in the regulation of oxygen evolution and stabilizes the oxygen evolving complex of PSII.

  Synonyms:

PsbP

  Immunogen:

KLH-conjugated synthetic peptide of PsbP derived from Arabidopsis thaliana AT1G06680, AT2G30790.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at arashare01@qq.com

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

28 kDa (AT1G06680), 13 kDa (AT2G30790)

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica napus, Vitis vinifera, Gossypium raimondii, Brassica rapa, Glycine max, Cucumis sativus, Populus trichocarpa, Medicago truncatula, Sorghum bicolor, Setaria viridis, Solanum tuberosum, Hordeum vulgare, Triticum aestivum, Solanum lycopersicum, Zea mays, Oryza sativa, and 80-99% homologues with the sequence in Nicotiana tabacum, Physcomitrella patens.
For more species homologues information, please contact tech support at arashare01@qq.com

 

Application Example

1-2 is thylakoid membrane protein from Arabidopsis thaliana leaf containing 1.25 μg, 2.5 μg of chlorophyll, respectively.
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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