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PsbW / Anti-PsbW subunit of Photosystem II antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY2421S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

PsbW is a nuclear-encoded PSII subunit and found in higher plants but not in cyanobacteria. PsbW has an apparent molecular mass of 6.1 kDa containing 54 amino acids. Loss of PsbW destabilizes the supramolecular organization of PSII.

  Synonyms:

PsbW, Photosystem II reaction center W protein, chloroplastic, PSII 6.1 kDa protein

  Immunogen:

KLH-conjugated synthetic peptide derived from C-terminal section of PsbW protein in Arabidopsis thaliana AT2G30570.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at arashare01@qq.com

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

14 / 9 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Hordeum vulgareSorghum bicolorMedicago truncatulaSpinacia oleraceaNicotiana tabacumVitis viniferaPopulus trichocarpaSetaria viridisSolanum lycopersicumSolanum tuberosumBrassica rapaZea maysTriticum aestivumBrassica napusGossypium raimondiiOryza sativa Japonica Group, and 80-99% homologues with the sequence in Physcomitrella patensCucumis sativusGlycine max.
For more species homologues information, please contact tech support at arashare01@qq.com

 

Application Example

1-3 is thylakoid membrane protein from WT of Arabidopsis thaliana leaf containing 1.25 μg, 2.5 μg, and 5 μg of chlorophyll, respectively. 
Electrophoresis: Tricine-SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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