Product Information
Description: | Rabbit polyclonal antibody |
Background: | The plant 20S proteasome β subunit 1 (PBA1) was shown to have caspase-3-like activity using biotin-DEVD-fmk pull-down and activity assay in downregulated lines. |
Synonyms: | PBA1 |
Immunogen: | KLH-conjugated synthetic peptide derived from N-terminal section of PBA1 protein in Arabidopsis thaliana AT4G31300. |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Serum |
Reconstitution: | Reconstitution with 150 µl of sterile water. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Recommended Dilution: | Western Blot (1:1000-1:5000) |
Expected/apparent MW: | 25 / 26 kDa |
Confirmed Reactivity: | Arabidopsis thaliana |
Predicted Reactivity: | Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Nicotiana tabacum, Solanum lycopersicum, Solanum tuberosum, Brassica rapa, Cucumis sativus, Brassica napus, Medicago truncatula, Physcomitrium patens, and 80-99% homologues with the sequence in Spinacia oleracea, Vitis vinifera, Gossypium raimondii, Populus trichocarpa. |
Application Example
15 μl cytosolic protein from Arabidopsis thaliana leaf. |