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PPH / Anti-Pheophytinase, chloroplastic antibody
价格:¥2540.00 ¥3180.00
积分:150 分
货号:PHY2535A
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

Chlorophyll (Chl) degradation occurs naturally during leaf maturation and senescence, and can be induced by stresses, both processes involving the regulation of plant hormones. PPH is a pheophytinase that is involved in chlorophyll breakdown.

  Synonyms:

PPH, CO-REGULATED WITH NYE1, CRN1, PHEOPHYTINASE

  Immunogen:

KLH-conjugated synthetic peptide of PPH derived from Arabidopsis thaliana AT5G13800.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150µl of 0.01 M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:10000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

55 / 54 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity:  

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica rapaBrassica napus, and 80-99% homologues with the sequence in Vitis viniferaPopulus trichocarpaGossypium raimondiiGlycine maxNicotiana tabacumSolanum lycopersicumSolanum tuberosumCucumis sativusMedicago truncatula.
For more species homologues information, please contact tech support at arashare01@qq.com.

 

Application Example

40 μg total chloroplast protein from Arabidopsis thaliana leaf.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:10000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.