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RPN1 / Anti-26S proteasome non-ATPase regulatory subunit 2 homolog A/B antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY2594S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

The 26S proteasome plays a central role in the degradation of regulatory proteins involved in a variety of developmental processes. It consists of two multisubunit protein complexes: the proteolytic core protease and the regulatory particle (RP). The RPN1 subunit belongs to the base complex of the RP and is known to physically interact with two proteins carrying ubiquitin-like domains, Rad23 and Dsk2.

  Synonyms:

RPN1

  Immunogen:

KLH-conjugated synthetic peptide of RPN1 derived from Arabidopsis thaliana AT2G20580, AT4G28470.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at arashare01@qq.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:5000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

98 / 95 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Zea maysBrassica rapaVitis viniferaOryza sativa Japonica GroupNicotiana tabacumHordeum vulgareSolanum tuberosumSolanum lycopersicumCucumis sativusSpinacia oleraceaSorghum bicolor, Brassica napusSetaria viridisPopulus trichocarpaMedicago truncatulaGlycine max, and 80-99% homologues with the sequence in Gossypium raimondii.
For more species homologues information, please contact tech support at arashare01@qq.com.

 

Application Example

Lane 1: 10 μl cytosolic protein from Arabidopsis thaliana leaf.
Lane 2: 6 μg nuclear protein from Arabidopsis thaliana.
Electrophoresis: 10% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:5000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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