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LPA3 / Anti-Protein LOW PSII ACCUMULATION 3, chloroplastic antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY2617S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

Photosystem II (PSII) is a multisubunit membrane protein complex that is assembled in a sequence of steps. Low PSII Accumulation 3 (LPA3) is required for the assembly of the CP43 subunit in PSII complexes in Arabidopsis. It interacts with LPA2, a previously identified PSII CP43 assembly factor, and a double mutation of LPA2 and LPA3 is more deleterious for assembly than either single mutation, resulting in a seedling-lethal phenotype.

  Synonyms:

LPA3, LOW PSII ACCUMULATION 3

  Immunogen:

KLH-conjugated synthetic peptide of LPA3 derived from Arabidopsis thaliana AT1G73060.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at arashare01@qq.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:5000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

40 / 33 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Cucumis sativus, Populus trichocarpa, and 80-99% homologues with the sequence in Gossypium raimondii, Vitis vinifera, Brassica napus, Brassica rapa, Spinacia oleracea, Zea mays, Hordeum vulgare, Setaria viridis, Sorghum bicolor, Glycine max, Oryza sativa Japonica Group.
For more species homologues information, please contact tech support at arashare01@qq.com.

 

Application Example

4 μg total chloroplast protein from Arabidopsis thaliana leaf.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:5000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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