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AT1G07040 / Anti-AT1G07040 protein antibody
价格:¥2540.00 ¥3180.00
积分:150 分
货号:PHY2656A
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

AT1G07040

  Synonyms:

AT1G07040

  Immunogen:

KLH-conjugated synthetic peptide of AT1G07040 protein derived from Arabidopsis thalianaAT1G07040.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150 µl of 0.01 M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

41 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Vitis vinifera, Zea mays, Physcomitrium patens, Solanum lycopersicum, Brassica napus, Nicotiana tabacum, Brassica rapa, Solanum tuberosum, Populus trichocarpa, Gossypium raimondii, Cucumis sativus, Oryza sativa, Oryza sativa Japonica Group, Sorghum bicolor, Setaria viridis, Hordeum vulgare subsp. vulgare, and 80-99% homologues with the sequence in Spinacia oleracea.
For more species homologues information, please contact tech support at arashare01@qq.com.

 

Application Example

80 μg total chloroplast protein from Arabidopsis thaliana leaf.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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