返回首页 登录 注册 购物车
关于我们 通知公告 服务指南 联系我们
试剂耗材频道
搜产品 敬请关注公众号
总站导航 AraShare PlantShare ExpShare 植科头条 学术讲堂 求职招聘 仪器设备 试剂耗材 技术服务 试剂耗材 植物抗体 拟南芥抗体
SKL2 / Anti-Probable inactive shikimate kinase like 2, chloroplastic antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY2684S
数量:999
- +
加入购物车 立即购买 收藏此商品

Product Information

  Description:

Rabbit polyclonal antibody

  Background:

SKL2 does not possess shikimate kinase activity in vitro probably because it lacks the conserved active sites and substrate binding sites of the shikimate kinase family.

  Synonyms:

SKL2, SHIKIMATE KINASE-LIKE 2

  Immunogen:

KLH-conjugated synthetic peptide of SKL2 derived from Arabidopsis thaliana AT2G35500.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at arashare01@qq.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:5000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

43 / 34 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity:  

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica napus, Brassica rapa, Cucumis sativus, Gossypium raimondii, Medicago truncatula, Glycine max, and 80-99% homologues with the sequence in Solanum lycopersicum, Solanum tuberosum, Nicotiana tabacum, Vitis vinifera, Spinacia oleracea, Hordeum vulgare, Zea mays, Sorghum bicolor, Setaria viridis, Triticum aestivum, Oryza sativa Japonica Group.
For more species homologues information, please contact tech support at arashare01@qq.com.

 

Application Example

5 μl total chloroplast protein from Arabidopsis thaliana leaf. 
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:5000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


猜你喜欢 CSD2 / Anti-Ch...
¥ 2540.00 ¥ 3180.00
AtpB / Anti-Be...
¥ 3100.00 ¥ 3880.00
Arf1 /Anti-ADP...
¥ 2310.00 ¥ 2890.00
Actin / Anti-A...
¥ 3100.00 ¥ 3880.00
CSD2 / Anti-Ch...
¥ 2540.00 ¥ 3180.00
AtpB / Anti-Be...
¥ 3100.00 ¥ 3880.00
Arf1 /Anti-ADP...
¥ 2310.00 ¥ 2890.00
Actin / Anti-A...
¥ 3100.00 ¥ 3880.00