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HEME2 / Anti-Uroporphyrinogen decarboxylase 2, chloroplastic antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY2721S
数量:999
- +
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

HEME2 catalyzes the decarboxylation of four acetate groups of uroporphyrinogen-III to yield coproporphyrinogen-III.

  Synonyms:

HEME2

  Immunogen:

KLH-conjugated synthetic peptide derived from C-terminal section of HEME2 protein in Arabidopsis thaliana AT2G40490.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at arashare01@qq.com.

  Reconstitution:  

Reconstitution with 150 µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:5000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

44 / 40 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity:  

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Zea maysPopulus trichocarpa, Hordeum vulgare, Setaria viridis, Sorghum bicolor, Nicotiana tabacum, Spinacia oleracea, Cucumis sativus, Solanum tuberosum, Brassica rapa, Brassica napus, Oryza sativa Japonica Group, Solanum lycopersicum, Glycine max, Oryza sativa Indica Group, and 80-99% homologues with the sequence in Vitis vinifera, Gossypium raimondii, Medicago truncatula.
For more species homologues information, please contact tech support at arashare01@qq.com.

 

Application Example

15 μg stromal protein from Arabidopsis thaliana leaf. 
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:5000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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