Product Information
Description: | Rabbit polyclonal antibody |
Background: | Ascorbate peroxidases are enzymes that scavenge hydrogen peroxide in plant cells. Eight types of APX have been described for Arabidopsis: three cytosolic (APX1 (AT1G07890), APX2 (AT3G09640), APX6 (AT4G32320)), two chloroplastic types (stromal sAPX (AT4G08390), thylakoid tAPX (AT1G77490)), and three microsomal (APX3 (AT4G35000), APX4 (AT4G09010), APX5 (AT4G35970)) isoforms. |
Synonyms: | TAPX, THYLAKOIDAL ASCORBATE PEROXIDASE |
Immunogen: | KLH-conjugated synthetic peptide derived from C-terminal section of TAPX protein in Arabidopsis thaliana AT1G77490. |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Serum |
Reconstitution: | Reconstitution with 150 µl of sterile water. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Recommended Dilution: | Western Blot (1:1000-1:5000) |
Expected/apparent MW: | 46 / 38 kDa |
Confirmed Reactivity: | Arabidopsis thaliana |
Predicted Reactivity: | Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Cucumis sativus, Hordeum vulgare subsp. vulgare, Triticum aestivum, Brassica rapa, Brassica napus, Nicotiana tabacum, Glycine max, Vitis vinifera, Populus trichocarpa, Solanum lycopersicum, Solanum tuberosum, Setaria viridis, and 80-99% homologues with the sequence in Oryza sativa Japonica Group, Medicago truncatula, Gossypium raimondii, Sorghum bicolor. |
Application Example
Lane 1: 80 μg total chloroplast protein from Arabidopsis thaliana leaf. |