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SHM1/2 / Anti-Serine hydroxymethyltransferase 1/2, mitochondrial antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY2884S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

Serine hydroxymethyltransferases (SHMs) are important enzymes of cellular one-carbon metabolism and are essential for the photorespiratory glycine-into-serine conversion in leaf mesophyll mitochondria. SHM1 catalyzes the conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. And SHM2 is thought to be localized in the mitochondrial matrix.

  Synonyms:

SHM1/2

  Immunogen:

KLH-conjugated synthetic peptide of SHM1/2 derived from Arabidopsis thaliana AT4G37930, AT5G26780.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at arashare01@qq.com.

  Reconstitution:  

Reconstitution with 150 µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

57 kDa (AT4G37930), 59 kDa (AT5G26780)

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity:  

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica rapa, Nicotiana tabacum, Oryza sativa Japonica Group, Physcomitrium patens, Chlamydomonas reinhardtii, Populus trichocarpa, Spinacia oleracea, Solanum tuberosum, Brassica napus, Cucumis sativus, Glycine max, Solanum lycopersicum, Vitis vinifera, Medicago truncatula, Gossypium raimondii, Setaria viridis, Zea mays, Sorghum bicolor, Hordeum vulgare.
For more species homologues information, please contact tech support at arashare01@qq.com.

 

Application Example

20 μg total protein from Arabidopsis thaliana leaf.
Electrophoresis: 12% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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