Product Information
Description: | Rabbit polyclonal antibody |
Background: | Serine hydroxymethyltransferases (SHMs) are important enzymes of cellular one-carbon metabolism and are essential for the photorespiratory glycine-into-serine conversion in leaf mesophyll mitochondria. SHM1 catalyzes the conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. And SHM2 is thought to be localized in the mitochondrial matrix. |
Synonyms: | SHM1/2 |
Immunogen: | KLH-conjugated synthetic peptide of SHM1/2 derived from Arabidopsis thaliana AT4G37930, AT5G26780. |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Serum |
Reconstitution: | Reconstitution with 150 µl of sterile water. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Recommended Dilution: | Western Blot (1:1000-1:2000) |
Expected/apparent MW: | 57 kDa (AT4G37930), 59 kDa (AT5G26780) |
Confirmed Reactivity: | Arabidopsis thaliana |
Predicted Reactivity: | Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica rapa, Nicotiana tabacum, Oryza sativa Japonica Group, Physcomitrium patens, Chlamydomonas reinhardtii, Populus trichocarpa, Spinacia oleracea, Solanum tuberosum, Brassica napus, Cucumis sativus, Glycine max, Solanum lycopersicum, Vitis vinifera, Medicago truncatula, Gossypium raimondii, Setaria viridis, Zea mays, Sorghum bicolor, Hordeum vulgare. |
Application Example
20 μg total protein from Arabidopsis thaliana leaf. |